Called Rebecca, the media provides enumeration of both organisms in 24 hours using just one single plate and without any confirmation. By contrast, standard methods recommend the use of up to two plates per dilution and for each organism with confirmation of the presumptive positives.
Recently validated and approved by AFNOR to ISO 16140 standard, the Rebecca method can be used with both food and animal feed. The procedure is carried out in just two steps – plating and reading – and requires no confirmation or specialised equipment. It can be used for single enumeration of E.Coli or simultaneous enumeration of E.coli and Enterobacteriaceae using a single plate. After a 24-hour incubation step, E.Coli will appear as blue colonies and the other Enterobacteria will appear as pink to red due to a specific chromogenic substrate.
AES-Chemunex says this simple method offers significant workload and cost savings compared with standard culture methods. Technicians only have to use a single plate for a simultaneous enumeration of the organisms, which minimises media consumption, workload, handling, saves on lab space and reduces the volume of waste and the cost of the analysis. www.aeschemunex.com