New assays for easy detection of HAIs

Published: 20-Nov-2008

Q Chip Ltd, a leading Cardiff-based developer of microencapsulation solutions, has introduced ReaX beads for the detection of bacterial and viral hospital acquired infections (HAIs).


The product portfolio currently includes assays for MRSA, Klebsiella and Pseudomonas with tests for norovirus, Clostridium difficile and Vancomycin Resistant Enterococcus (VRE) scheduled for launch early next year.

A single bead is a complete PCR assay in an extremely easy-to-use format with a much simplified workflow compared to some currently available molecular diagnostic tests.

The ReaX MRSA bead contains a multiplex end-point PCR assay to identify antibiotic (mecA) resistant Staphylococcus aureus. The ReaX beads for Klebsiella and Pseudomonas contain all the assay components including the probes & primers for a real time PCR assay. The format for the norovirus assay is a two-step RT PCR assay consisting of two beads: an RT ReaX bead and a qPCR bead.

Use of ReaX reduces both set up times and errors caused by pipetting and enable less skilled operators to perform complex molecular assays. Compared with standard assay protocols, Q Chip says the beads increase test reproducibility and reduce false negative and false positive results.

The ReaX format offers the first real alternative to lyophilisation as a stability technology for molecular applications. The beads are produced from hydrogel polymers in Q Chip’s proprietary MicroPlant microfluidic device.

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