In nature and in process systems, micro-organisms rarely exist as single cells or even as pure cultures, but rather as a monoculture or a mixed culture of different micro-organisms. Micro-organisms within a biofilm, such as Pseudomonas species, are commonly encased in a slimy matrix referred to as extracellular polymeric substances (EPS), which is essential for the micro-organisms’ survival.
The development and presence of the EPS is very important in increasing the micro-organisms’ resistance to environmental stresses, antimicrobial agents and cleaning agents. Therefore it is critical to remove the EPS prior to sanitisation or disinfection. Surface conditions, such as rouge, can also play a role in removal and disinfection so it is important to consider these when developing a remediation strategy.