Testing for e.coli
A new test for the simultaneous detection of coliforms and enumeration of e.coli in food products which brings significant advantages over traditional methods for detecting coliforms and e.coli is now available from Schleicher & Schuell Microscience. MI BLUE Agar is a rapid test providing a quick turn around delivering results within a 6 to 8 hour time span. The test detects the presence of the bacterial enzymes b-Galactosidase and b-Gluucoronidase produced by coliforms and e.coli, respectively. Coliforms produce fluorescent colonies when exposed to long-wave UV-light while coliforms other than e.coli show blue-white fluorescence; e.coli colonies are blue in colour with blue to blue-green fluorescence and may exhibit a fluorescent halo. E.coli produces blue colonies on MI Blue under ambient light due to the breakdown of IBDG by the bacterial enzyme b-Glucurondase. This test enables blue e.coli colonies to be counted on plates with heavy particulates or high concentrations of total bacteria, without the need for an additional confirmation test or the expense of other capital equipment. MI Blue Agar requires just one incubation temperature of 42?C and provides a true quantitative method of a true count versus a statistical most probable number value.
T: +44(0)20 8361 3111 salesuk@schleicher-schuell.de