DSMZ houses public collection of spacecraft-associated bacteria

Spacecraft are assembled in cleanrooms under the strict controls for bio-contamination as once sent into space the risk of biological contamination of other planets is high. Space agencies have defined standards by which to measure the bioburden and diversity of microbial species in the cleanrooms and on the spacecraft. The European Space Agency (ESA) is funding a collection of microbial strains from spaceflight hardware and associated assembly facilities at the DSMZ. This collection is an important resource for research institutes and industry to investigate adaptive mechanisms of bacteria (for instance, resistance to heat, UV radiation, ionizing radiation, desiccation, disinfectants).

Sample taking the ESA Herschel space observatory (ESA/DLR)



Bacteria are ubiquitous and some of them are real survival specialists – a property, which is particularly challenging for space missions. The spacecraft that are sent on their long journey into space should be as clean as possible and considerably reduced in microbial burden, since the risk of biological contamination of other planets is high. Such a contamination could affect the detection of extraterrestrial life or make it even impossible.

For this reason, spacecraft are assembled in under the most stringent cleanroom controls for bio-contamination. Nevertheless, micro-organisms exist that can deal with the prevailing extreme conditions such as dryness, lack of nutrients or presence of disinfectants. Space agencies have defined standards by which to measure the bioburden and diversity of microbial species in thecleanrooms and on the spacecraft.

The Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ) now offers, in cooperation with the European Space Agency (ESA), the first public collection of "extremotolerant" bacterial strains adapted to the harsh conditions within cleanrooms. This collection is an important resource for research institutes and industry to investigate adaptive mechanisms of bacteria (for instance, resistance to heat, UV radiation, ionising radiation, desiccation, disinfectants).

The journal Astrobiology reports about this culture collection in its current issue.1

"For any space mission an upper limit of bioburden is defined," says Dr Rüdiger Pukall, a microbiologist at DSMZ. "Bioburden measurements are a crucial part of Planetary Protection requirements”. This concept includes all activities that prevent contamination of planets and other celestial bodies by terrestrial forms of life, such as micro-organisms, in the context of inter-planetary space missions. It is of particular importance to evaluate the biodiversity of microbial communities on spaceflight hardware and in associated assembly facilities.

The objective in doing so is to develop appropriate decontamination strategies to avoid microbial hitchhikers during the next mission to Mars." Selected surface areas within European spacecraft associated cleanrooms as well as the surface of the Herschel Space Observatory located therein were sampled between 2007 until 2009 by microbiologists from the Leibniz-Institute DSMZ, the German Aerospace Center (DLR) and the University Regensburg.

A cleanroom is a particularly extreme habitat for microbial survivalists

The Herschel Space Observatory was constructed at various locations around the globe: cleanrooms in Friedrichshafen (Germany), Noordwijk (Netherlands), and at Europe’s spaceport in Kourou (French Guiana).

"A cleanroom is a particularly extreme habitat for microbial survivalists", explains Rüdiger Pukall. "The nutrient-poor environment, controlled moisture and temperature, air filtering and frequent decontamination of surfaces create a special habitat for spore-forming, autotrophic, multi-resistant, facultatively or obligate anaerobic bacteria."

Even taking a sample of the bacteria in the cleanrooms is a special challenge for the researchers. "In order not to introduce any foreign micro-organisms or particles, the microbiologists have to wear protective suits and face masks", reports Rüdiger Pukall. “Here the samples were taken with special swabs and wipes, according to defined standard procedures from ESA. The samples were subsequently analysed at the University Regensburg and the DLR in Köln and the bacteria were isolated using diverse cultivation strategies."


Phylogenetic diversity of isolates currently available in the culture collection (Moissl-Eichinger et. al, Astrobiology, Vol.12, 11, 2012)


A collection of "survivalists"

The core of this special collection consists of about 300 bacterial strains that were isolated from various cleanrooms. All bacteria belong to Risk group 1 or 2. A large portion of the isolates can be assigned to the Gram-positive bacteria, whereby spore-forming bacteria from the species Bacillus as well as Micrococcus and Staphylococcus species are represented. Gram-negative bacteria are predominantly represented by the species Acinetobacter, Pseudomonas and Stenotrophomonas. Recently, an additional set of 60 isolates affiliated to these genera were added to the ESA collection. The isolates derived from samples taken in 2003 and 2004 within an ESA founded project in co-operation of DLR and DSMZ during preparation of the missions SMART-1 (interplanetary satellite, lunar mission) and ROSETTA (exploration of comets) in Noordwijk and Kourou. Five cleanroom isolates were provided by the NASA Jet Propulsion Laboratory (USA) in addition. About 30% of the microbes within the ESA collection are still unknown and have now been made available for research purposes. Some of these isolates have been described recently as a novel species, such as Paenibacillus purispatii(DSM 22991) or Tersicoccus phoenicis(KO_PS43, DSM 30849), a representative of a new bacterial genus.

The collection of extremotolerant microbes, which are adapted to the artificial biotope of the cleanrooms, offers an extraordinary valuable and beneficial source for industry and research. For ESA, the culture collection is an essential tool to understand the biological contamination and its potential risk and to evaluate novel biological decontamination procedures and disinfection strategies.

The collection will be expanded within the next three years by including more extremotolerant bacteria that could be of interest for industry and research.

Link to the collection: www.dsmz.de/catalogues/catalogue-microorganisms/specific-catalogues/esa-strains.html

References

1. Moissl-Eichinger C, Rettberg P, Pukall R. (2012). The first collection of spacecraft-associated microorganisms: a public source for extremotolerant microorganisms from spacecraft assembly clean rooms. Astrobiology 12(11):1024-1034.

2. Stieglmeier M, et al, (2012). Abundance and diversity of microbial inhabitants in European spacecraft-associated cleanrooms. Astrobiology 12(6):572-585.

Additional literature:

1.Behrendt U, et al, Syst Appl Microbiol. 2010 Oct; 33(6):328-36.

2.Vaishampayan P, et al, (2012). Int J Syst Evol Microbiol. 2012 Dec 7. (Epub ahead of print)